ABSTRACT
Although most acute myeloid leukemia (AML) patients can achieve complete remission (CR) induced by standardized chemotherapy, but the relapse rate after remission remains high. The key reason is its high heterogeneity in cytogenetics and molecular biology. There are evidences show that minimal residual disease (MRD) is closely associated with disease recurrence, so that, finding specific genetic and molecular biological changes as new targets for MRD detection has become a research hotspot in recent years. In this review the intrinsic relationship between relapse of AML and MRD detection of specific molecular events, the application of these new targets in MRD detection and their targeted therapies according to the latest guidelines, so as to achieve the optimal treatment in CR phase.
Subject(s)
Humans , Flow Cytometry , Leukemia, Myeloid, Acute , Neoplasm, Residual , Prognosis , Recurrence , Remission InductionABSTRACT
The aim of study was to investigate the regulatory effect of endomorphin-1 (EM-1) on ICAM-1 and VCAM-1 on normal human bone marrow stromal cell surface as well as IL-6 and TNF-α excreted by human normal bone marrow stromal cells. The expression of ICAM-1 and VCAM-1 was determined by flow cytometry, and the concentration of IL-6 and TNF-α was detected by ELISA. The results indicated that the expression of ICAM-1 and VCAM-1 was significantly different from the control group after being cultured with endomorphin-1 for 24 hours (p < 0.05), and different concentrations of endomorphin-1 had no effect on expressions of ICAM-1 and VCAM-1 both; there was no difference in secretion of IL-6 and TNF-α between experimental and control groups (p > 0.05). It is concluded that endomorphin-1 regulates the ICAM-1 and VCAM-1 expression on human normal bone marrow stromal cell surface, but endomorphin-1 does not display any effect on IL-6 and TNF-α secreted by human normal bone marrow stromal cells.
Subject(s)
Humans , Bone Marrow Cells , Cell Biology , Metabolism , Cell Line , Hematopoietic System , Metabolism , Intercellular Adhesion Molecule-1 , Metabolism , Interleukin-6 , Metabolism , Mesenchymal Stem Cells , Cell Biology , Metabolism , Oligopeptides , Pharmacology , Tumor Necrosis Factor-alpha , Metabolism , Vascular Cell Adhesion Molecule-1 , MetabolismABSTRACT
Objective To explore the diagnosis and curative effect of phenylketonuria(PKU) in Gansu.Methods Fifty-nine thousand nine hundred infants and 118 borderline cases were screened from Oct.1999 to Dec.2006.Three drops of venous blood was collected from the exterior or interior part in the heel,and trickled the venous blood onto the specialized filter paper,which could be formed into 3 bloodstains with the diameter of 0.8-1.0 cm.Phenylalanine(phe) was detected by chemiluminescence method,and PKU was screened by the PKU standard of diagnosis.Infants with PKU had been given low phe food.During the treatment,phe level,physical development and intellectual development were measured regularly.Patients′ body weight,height and head circumference were measured once 1 month.Patients′ intellectual quotient(IQ) were measured every half year(IQ was analyzed by Gesell test).Results were analyzed by SPSS 11.0 software for t test.Results In total,93 patients with PKU were diagnosed and treated.Thirty-six cases were identified by neonatal screening before 3 mouths old.Mental retardation was found in 57 cases before 6 years old.After treatment with low-phe diet,the follow-up for early-treated patients revealed that their physical and mental development were normal,and height,weight and head circumference were in the normal amplitude,and the results of IQ was(92?12)scores.In later treated patients,abnormal behaviors were significantly improved and their developmental quotients were elevated.Before treatment,Gesell value was(57?20)scores;after 12-18 months treatment Gesell value was(70?20)scores.The difference was significant(t=1.705 P
ABSTRACT
The study was aimed to explore the possible association between HLA-DRB1 allele polymorphism and the susceptibility to leukemia in Gansu Chinese Han nationality and to find the genes susceptible to leukemia. HLA-DRB1 genes in 74 patients with leukemia from northwestern China and 82 healthy Chinese controls were determined by polymerase chain reaction and sequence-specific oligonucleotide probe hybridizations (PCR/SSO) DNA analysis. The results showed that as compared with control, the allele frequencies of HLA-DRB1*03 (chi(2) = 8.125, P = 0.004), HLA-DRB1*07 (chi(2) = 13.526, P = 0.000), HLA-DRB1*08 (chi(2) = 18.855, P = 0.000), HLA-DRB1*13 (chi(2) = 7.039, P = 0.008) significantly increased in AML group. The allele frequencies of HLA-DRB1*07 (chi(2) = 5.689, P = 0.017), HLA-DRB1*11 (chi(2) = 7.73, P = 0.005), HLA-DRB1*12 (chi(2) = 4.234, P = 0.040), HLA-DRB1*13 (chi(2) = 38.333, P = 0.000) significantly increased in CML group. The allele frequency of HLA-DRB1*01 (chi(2) = 5.294, P = 0.021) significantly increased in ALL group. It is concluded that the susceptibility to AML in Gansu Han nationality is positively related to HLA-DRB1*03. 1*07.1*08.1*13. CML positively correlates with HLA-DRB1*07. 1*11.1*12.1*13, and ALL may be positively in relation with HLA-DRB1*01. Allele polymorphism is associated with the leukemia occurrence.